How do you make a cytology smear?

How is smear prepared for cytology?

The cytologic smears were im- munostained within 24 hours of preparation. The wet-fixed smears were prepared by immersing the smears immediately in 95% ethanol before they dried. The air-dried smears were prepared by al- lowing the smeared material to dry completely on the slides.

How do you prepare cell samples for cytological study?

In the cytology laboratory various sample preparation steps are performed prior to analysis including centrifugation to concentrate cells in dilute samples, followed by preparation and staining of slides, and examination under a light microscope (Figure 1).

How do you collect a cytology sample?

Cytology samples can be collected from solid lesions by several techniques including:

  1. Aspiration cytology – fine needle aspiration (FNA): Ideal for cutaneous or subcutaneous masses since it avoids surface contamination. …
  2. Exfoliative cytology:

How do you make a smear?


  1. Place one needle of solid bacterial growth or two loops. of liquid bacterial growth in the center of a clean slide.
  2. If working from a solid medium, add one drop (and only one drop) …
  3. Now, with your inoculating loop, mix the specimen with the water. …
  4. Place the slide on a slide warmer and wait for it to dry.
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What are the methods of smear preparation?

Four different types of smear preparation methods (conventional method, blood film method, drop and rest method, and water-wash method) were carried out according to the standard reference as described below.

How do you do a smear swab test?

Use a saline moistened swab (sterile saline, not water, as the osmotic effect of water ruptures the cells) to remove superficial debris. If bloody, swab/ dab with clean paper towel to remove blood first. Gently touch the tissue surface to a clean slide. Repeat touch imprint along the length of the slide.

How do you make a CSF slide?

Slide preparation of cerebrospinal fluid for cytological examination

  1. 1) Immediate processing of the liquor samples. …
  2. 2) Excellent preparation quality. …
  3. 1) Sample preparation. …
  4. 3) Assembly of the cyto insert. …
  6. a) Sedimentation. …
  7. b) Removal of the cell-free supernatant. …
  8. c) Drying of the sediment.

What are cytological techniques?

Cytological techniques are methods used in the study or manipulation of cells. These include methods used in cell biology to culture, track, phenotype, sort and screen cells in populations or tissues, and molecular methods to understand cellular function.

What is a cytology smear?

A cervical cytology (cervical smear) sample is a sample of cells from the outside of the cervix (or neck of the womb) that allows detection of pre-cancerous abnormalities of the cervix. These changes can then be treated successfully before cancer develops.

What fixative can be used for cytology smears?

Ethyl alcohol (95%) is the most commonly used fixative in cytology. The cytology samples are processed commonly by direct smear, centrifugation, cytocentrifugation, liquid-based preparation, Millipore technique and cell block.

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What is cytological fixative?

Cytology Fixative covers cells with a tough, soluble film that protects cell morphology for microscopic examination. It is water and alcohol soluble, environmentally friendly and extremely economical.

What are the 3 types of specimens for Pap smear?

Acceptable specimens for the Pap Test include cervical, cervical and vaginal, endocervical, vaginal and/or vulvar scrapings or brushings. Specimens may be submitted as conventional smears, although the preferred method is the ThinPrep® Pap test.

What are the stains used in cytology?

The universal stain for cytological preparations is the Papanicolaou stain. Harris’ hematoxylin is the optimum nuclear stain and the combination of OG6 and EA50 give the subtle range of green, blue and pink hues to the cell cytoplasm.

What is the difference between biopsy and cytology?

A cytology test is different from a biopsy. During a biopsy, tissue from a certain area of the body is removed and analyzed for cancer. A cytology test removes and studies a fewer number of cells. With a cytology test, the cytological morphology of the cells collected are studied under a microscope.