Which base of DNA binds to CIS Platin in cancer treatment?

Cisplatin binds to the N7 reactive center on purine residues and as such can cause deoxyribonucleic acid (DNA) damage in cancer cells, blocking cell division and resulting in apoptotic cell death. The 1,2-intrastrand cross-links of purine bases with cisplatin are the most notable among the changes in DNA.

How do platinum anticancer drugs bind to DNA?

Platinum-containing molecules are widely used as anticancer drugs. These molecules exert cytotoxic effects by binding to DNA through various mechanisms. … In particular, relevant differences in DNA stability at the single-molecule level are demonstrated by analyzing nanomechanically induced DNA denaturation.

What DNA base does cisplatin bind to?

Cisplatin has been demonstrated to bind to the purine-N(7) of guanine and adenine residues on DNA, mainly forming 1,2-intrastrand crosslinks between adjacent two guanines (1,2-d(GpG), ~65%) or neighboring adenine and guanine (1,2-d(ApG), ~25%), as well as interstrand crosslinks and mono-functional adduct [7,8,9,10].

How does Platin treat cancer?

Platinum-based drugs are effective against cancer because at their centre is a platinum atom joined to two ammonion molecules and two chloride ions. The compound is negatively charged, but when it enters the cancer cell it becomes positively charged because the chloride ions are replaced by water molecules.

THIS IS IMPORTANT:  Can tires cause cancer?

How does cisplatin work in chemotherapy?

Cisplatin works by stopping the cancer cells from multiplying. It does this by binding together the strands of the cells’ genetic material, DNA. DNA is needed for growth and multiplication of cells. Cisplatin damages the DNA inside the cancer cells and so prevents them from multiplying.

What is the structure of Platin?

Cisplatin is a diamminedichloroplatinum compound in which the two ammine ligands and two chloro ligands are oriented in a cis planar configuration around the central platinum ion.

How is platinum-based chemotherapy administered?

Platinum drugs are taken into cells via a range of mechanisms, most notably copper transporters, and specifically high affinity copper transporter 1. Upon entering a cell, the low salt concentration results in the aquation of the drug and the loss of its labile groups (chlorides or carboxylate-based ligands).

What DNA bases does cisplatin have an affinity to coordinate and bind to?

The primary target of cisplatin is genomic DNA, specifically the N7 position of guanine bases. This point of attack first generates monofunctional adducts, which subsequently closes by coordination to the N7 position of an adjacent purine to afford an intrastrand cross-link.

Why does cisplatin binding to DNA prevent replication of the DNA molecule?

Cisplatin and other platinating agents are some of the most widely used chemotherapy agents. These drugs exert their antiproliferative effects by creating intrastrand and interstrand DNA cross-links, which block DNA replication.

Where does cisplatin bind to guanine?

Cisplatin has been demonstrated to bind to the purine-N(7) of guanine and adenine residues on DNA, mainly forming 1,2-intrastrand crosslinks between adjacent two guanines (1,2-d(GpG), ~65%) or neighboring adenine and guanine (1,2-d(ApG), ~25%), as well as interstrand crosslinks and mono-functional adduct [7,8,9,10].

THIS IS IMPORTANT:  Do benign bone tumors need to be removed?

What is the mode of action for the anthracycline anticancer agents?

Mechanism of Action

The most widely accepted explanation for the action of anthracyclines is their interaction with topoisomerase-II. The ternary complex thus formed prevents the re-ligation of the ds-DNA breaks. Subsequently, it promotes growth arrest and apoptotic cell death.

What is the mechanism of action of cyclophosphamide?

MECHANISM OF ACTION:

Cyclophosphamide is an alkylating agent of the nitrogen mustard type. 2 An activated form of cyclophosphamide, phosphoramide mustard, alkylates, or binds, to DNA. Its cytotoxic effect is mainly due to cross-linking of strands of DNA and RNA, and to inhibition of protein synthesis.

What is the mechanism of action of alkylating agents?

Most alkylating agents have similar mechanisms of action, but differ in their clinical efficacy. These agents act directly on DNA, resulting in its crosslinking and causing DNA strand breaks, leading to abnormal base pairing and inhibiting cell division, eventually resulting in cell death.